BxPC-3 cells were subjected to SDS PAGE followed by western blot with FNab05163(MGEA5 Antibody) at dilution of 1:1500
IP Result of anti-MGEA5 (IP:FNab05163, 3ug; Detection:FNab05163 1:500) with SH-SY5Y cells lysate 2900ug.
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using FNab05163(MGEA5 Antibody) at dilution of 1:200
Background
Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro)(PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity(PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro), but has about six times lower specific activity than isoform 1.